Poster or Presentation Title

Co-occurrence of Manganese oxidase genes

Location

Hall Memorial Ballroom

Access Type

Campus Access Only

Presentation Type

Poster Presentation

Start Date

4-4-2018 12:00 PM

Department

Biology

Abstract

In the environment, manganese exists mostly in three oxidation states, Mn(II), Mn(III) and Mn(IV). Manganese oxidizing bacteria play a role in the environmental availability of Manganese in different oxidation states. Pseudomonas putida GB-1 is a model organism for the study of manganese oxidation in bacteria, however, the frequency of co-localization of multiple known and suspected manganese oxidizing proteins, as well as their occurrence between species, is unknown. Eight different genes isolated from known manganese oxidizing bacteria (MnxG, MopA, McoA, PputGB1_2552, PputGB1_2553, MoxA, MofA, and Bacillus MnxG) were tested individually using BioPython and BLAST (Basic Local Alignment Search Tool) on multiple genomic databases. BLAST searches had an expect value cutoff of at least 1e-20, with most searches limited with an expect value cutoff of 1e-50. The data from these BLAST searches was compiled into multiple matrices and used to calculate gene correlation. In fellow members of gamma-proteobacteria, correlations between MnxG, MopA, McoA, PputGB1_2552, and PputGB1_2553 were high, while across all species and between MoxA, MofA, and Bacillus MnxG, correlations were low in exception to the correlation between PputGB1_2552 and PputGB1_2553. The correlation between PputGB1_2552 and PputGB1_2553 in all instances is incredibly high. Further research is being conducted to identify a correlation between genes and environment. These correlations can be used in future research to determine the role of suspected manganese oxidizing proteins.

Faculty Mentor

Katherine Geszvain, Mohamed Zaka Kurdi

Rights Statement

The right to download or print any portion of this material is granted by the copyright owner only for personal or educational use. The author/creator retains all proprietary rights, including copyright ownership. Any editing, other reproduction or other use of this material by any means requires the express written permission of the copyright owner. Except as provided above, or for any other use that is allowed by fair use (Title 17, §107 U.S.C.), you may not reproduce, republish, post, transmit or distribute any material from this web site in any physical or digital form without the permission of the copyright owner of the material.

This document is currently not available here.

Share

COinS
 
Apr 4th, 12:00 PM

Co-occurrence of Manganese oxidase genes

Hall Memorial Ballroom

In the environment, manganese exists mostly in three oxidation states, Mn(II), Mn(III) and Mn(IV). Manganese oxidizing bacteria play a role in the environmental availability of Manganese in different oxidation states. Pseudomonas putida GB-1 is a model organism for the study of manganese oxidation in bacteria, however, the frequency of co-localization of multiple known and suspected manganese oxidizing proteins, as well as their occurrence between species, is unknown. Eight different genes isolated from known manganese oxidizing bacteria (MnxG, MopA, McoA, PputGB1_2552, PputGB1_2553, MoxA, MofA, and Bacillus MnxG) were tested individually using BioPython and BLAST (Basic Local Alignment Search Tool) on multiple genomic databases. BLAST searches had an expect value cutoff of at least 1e-20, with most searches limited with an expect value cutoff of 1e-50. The data from these BLAST searches was compiled into multiple matrices and used to calculate gene correlation. In fellow members of gamma-proteobacteria, correlations between MnxG, MopA, McoA, PputGB1_2552, and PputGB1_2553 were high, while across all species and between MoxA, MofA, and Bacillus MnxG, correlations were low in exception to the correlation between PputGB1_2552 and PputGB1_2553. The correlation between PputGB1_2552 and PputGB1_2553 in all instances is incredibly high. Further research is being conducted to identify a correlation between genes and environment. These correlations can be used in future research to determine the role of suspected manganese oxidizing proteins.