Presenter Information

Chelsea ShultisFollow

Location

Schewel 232

Access Type

Campus Access Only

Presentation Type

Oral Presentation

Start Date

4-4-2018 10:45 AM

Department

Biology

Abstract

Nitric oxide (NO) is involved in many processes that occur throughout the human body, and it often indicates an immune response in the body due to inflammation. Common examples of processes that NO is involved in range from immune responses to the regulation of blood pressure. With nitric oxide production and health intertwined, accurately using this signaling molecule and testing the concentration of nitric oxide during physiological processes was raised. The aggregation of human platelets, a process that is part of the blood clotting process and is inhibited by NO release, allows scientists to use assay kits to measure NO concentration. We used a Griess reagent kit (Cayman Chemical, Ann Arbor Michigan) and collected blood from human volunteers. We then set out to determine if the Griess Reagent system could quantify nitric oxide production. Data that was collected was the average maximal percent aggregation as compared to the control we used. Our goal was to determine if the Griess reagent system is a tool that can be used to measure nitric oxide production accurately in living systems.

Faculty Mentor

Dr. Price Blair, Dr. Jason Crumpton, & Dr. Nancy Cowden

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Apr 4th, 10:45 AM

Quantifying Nitric Oxide Production in Platelets using the Griess Reagent System

Schewel 232

Nitric oxide (NO) is involved in many processes that occur throughout the human body, and it often indicates an immune response in the body due to inflammation. Common examples of processes that NO is involved in range from immune responses to the regulation of blood pressure. With nitric oxide production and health intertwined, accurately using this signaling molecule and testing the concentration of nitric oxide during physiological processes was raised. The aggregation of human platelets, a process that is part of the blood clotting process and is inhibited by NO release, allows scientists to use assay kits to measure NO concentration. We used a Griess reagent kit (Cayman Chemical, Ann Arbor Michigan) and collected blood from human volunteers. We then set out to determine if the Griess Reagent system could quantify nitric oxide production. Data that was collected was the average maximal percent aggregation as compared to the control we used. Our goal was to determine if the Griess reagent system is a tool that can be used to measure nitric oxide production accurately in living systems.