Presentations
Location
Schewel 232
Access Type
Campus Access Only
Entry Number
125
Start Date
4-4-2018 10:45 AM
Department
Biology
Abstract
Nitric oxide (NO) is involved in many processes that occur throughout the human body, and it often indicates an immune response in the body due to inflammation. Common examples of processes that NO is involved in range from immune responses to the regulation of blood pressure. With nitric oxide production and health intertwined, accurately using this signaling molecule and testing the concentration of nitric oxide during physiological processes was raised. The aggregation of human platelets, a process that is part of the blood clotting process and is inhibited by NO release, allows scientists to use assay kits to measure NO concentration. We used a Griess reagent kit (Cayman Chemical, Ann Arbor Michigan) and collected blood from human volunteers. We then set out to determine if the Griess Reagent system could quantify nitric oxide production. Data that was collected was the average maximal percent aggregation as compared to the control we used. Our goal was to determine if the Griess reagent system is a tool that can be used to measure nitric oxide production accurately in living systems.
Faculty Mentor(s)
Dr. Price Blair, Dr. Jason Crumpton, & Dr. Nancy Cowden
Rights Statement
The right to download or print any portion of this material is granted by the copyright owner only for personal or educational use. The author/creator retains all proprietary rights, including copyright ownership. Any editing, other reproduction or other use of this material by any means requires the express written permission of the copyright owner. Except as provided above, or for any other use that is allowed by fair use (Title 17, §107 U.S.C.), you may not reproduce, republish, post, transmit or distribute any material from this web site in any physical or digital form without the permission of the copyright owner of the material.
Quantifying Nitric Oxide Production in Platelets using the Griess Reagent System
Schewel 232
Nitric oxide (NO) is involved in many processes that occur throughout the human body, and it often indicates an immune response in the body due to inflammation. Common examples of processes that NO is involved in range from immune responses to the regulation of blood pressure. With nitric oxide production and health intertwined, accurately using this signaling molecule and testing the concentration of nitric oxide during physiological processes was raised. The aggregation of human platelets, a process that is part of the blood clotting process and is inhibited by NO release, allows scientists to use assay kits to measure NO concentration. We used a Griess reagent kit (Cayman Chemical, Ann Arbor Michigan) and collected blood from human volunteers. We then set out to determine if the Griess Reagent system could quantify nitric oxide production. Data that was collected was the average maximal percent aggregation as compared to the control we used. Our goal was to determine if the Griess reagent system is a tool that can be used to measure nitric oxide production accurately in living systems.