Presenter Information

T'onna CollinsFollow

Location

Schewel 232

Access Type

Open Access

Presentation Type

Oral Presentation

Start Date

4-4-2018 1:30 PM

Department

Biomedical Science

Abstract

The study’s focus is the manganese oxidation mechanism. This mechanism is crucial, especially in bacteria, because manganese is very active and is abundant in most of these organisms’ environments.Pseudomonas putida is the positive control that will be used to compare the DNA and amino sequences of specific genes in other organisms to see similarity and conservation of the mechanism. The top five organisms that is not part of the Pseudomonas genus and are similar to P. putida in each specific loci of the genes for manganese oxidation were found by a microbial database. These organisms were sequenced and aligned to see the similarities and differences of the manganese oxidation mechanism changes on an amino acid and DNA scale using Clustal Omega alignment tool . To physically see manganese oxidation, samples will be collected around Lynchburg College. The samples will be tested for the possibility of manganese oxidation. If they tested positive for the possibility, samples will be isolated to sequence the DNA and amino acids of the 16s ribosomal to rRNA gene, one of the genes known that is responsible for manganese oxidation. This gene was selected because it is highly conserved in bacteria for the mechanism. The goal is to see what genes are conserved from species to species in the manganese oxidation mechanism. This work is important because there is much to learn about the interaction of manganese and organisms.

Faculty Mentor

Dr. Christine Terry, Dr. Kati Geszvain, Dr. Nancy Cowden

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Apr 4th, 1:30 PM

Examining Manganese Oxidation in Bacteria Through Bioinformatics and Environmental Sampling

Schewel 232

The study’s focus is the manganese oxidation mechanism. This mechanism is crucial, especially in bacteria, because manganese is very active and is abundant in most of these organisms’ environments.Pseudomonas putida is the positive control that will be used to compare the DNA and amino sequences of specific genes in other organisms to see similarity and conservation of the mechanism. The top five organisms that is not part of the Pseudomonas genus and are similar to P. putida in each specific loci of the genes for manganese oxidation were found by a microbial database. These organisms were sequenced and aligned to see the similarities and differences of the manganese oxidation mechanism changes on an amino acid and DNA scale using Clustal Omega alignment tool . To physically see manganese oxidation, samples will be collected around Lynchburg College. The samples will be tested for the possibility of manganese oxidation. If they tested positive for the possibility, samples will be isolated to sequence the DNA and amino acids of the 16s ribosomal to rRNA gene, one of the genes known that is responsible for manganese oxidation. This gene was selected because it is highly conserved in bacteria for the mechanism. The goal is to see what genes are conserved from species to species in the manganese oxidation mechanism. This work is important because there is much to learn about the interaction of manganese and organisms.