Student Author Information

Mark StephensFollow

Access Type

Campus Access Only

Presentation Type

Oral Presentation

Start Date

April 2019

Department

Biomedical Science

Abstract

Feline oral cavities are diverse microbial environments which remain poorly examined. This is problematic because poor knowledge of oral diversity can lead to dental caries, gingivitis, and periodontal disease, without understanding their contributors. Understanding these contributors would provide better means of understanding pet oral health care for veterinarians. The oral microbiota of felines is rarely investigated for individual species or phylum-level taxonomic composition. In order to better understand the ecological and biochemical nature of feline oral cavities, isolated organisms and phyla need to be identified. Microbial isolates were collected by direct swab of gingival tissue. Swabs were grown overnight in Thioglycollate broth. Individual isolates were then grown on Columbia agar in aerobic and anaerobic conditions. Four isolates were retrieved for further testing. Preliminary identification suggests three Gram-positive cocci, and one Gram-positive rod. Biochemical and genomic techniques were used to isolate and identify individual bacterial species present. Previous work in our lab identified bacterial species such as Staphylococcus epidermidis, Bacillus subtilis, Kytococcus schroeteri, and others that profile in the genus Streptococcus or Neisseria.

Faculty Mentor(s)

David O. Freier, PhD

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Apr 10th, 8:30 AM

Genetic Identification and Biochemical Characterization of Bacterial Species from Feline Gingival Tissue

Feline oral cavities are diverse microbial environments which remain poorly examined. This is problematic because poor knowledge of oral diversity can lead to dental caries, gingivitis, and periodontal disease, without understanding their contributors. Understanding these contributors would provide better means of understanding pet oral health care for veterinarians. The oral microbiota of felines is rarely investigated for individual species or phylum-level taxonomic composition. In order to better understand the ecological and biochemical nature of feline oral cavities, isolated organisms and phyla need to be identified. Microbial isolates were collected by direct swab of gingival tissue. Swabs were grown overnight in Thioglycollate broth. Individual isolates were then grown on Columbia agar in aerobic and anaerobic conditions. Four isolates were retrieved for further testing. Preliminary identification suggests three Gram-positive cocci, and one Gram-positive rod. Biochemical and genomic techniques were used to isolate and identify individual bacterial species present. Previous work in our lab identified bacterial species such as Staphylococcus epidermidis, Bacillus subtilis, Kytococcus schroeteri, and others that profile in the genus Streptococcus or Neisseria.