Archived Abstracts

Poster or Presentation Title

Determining the Optimal Experimental Conditions for RAW 264.7 Cells

Location

Memorial Ballroom, Hall Campus Center

Access Type

Campus Access Only

Presentation Type

Poster Presentation

Start Date

8-4-2020 12:00 PM

End Date

8-4-2020 1:15 PM

Department

Biomedical Science

Abstract

RAW 264.7 cells are murine macrophages derived from mice that are commonplace in microbiological research. There is a notable lack of consistency for the experimental conditions chosen by researchers working with this cell line. This variability makes it difficult to compare the results of scientific literature involving RAW 264.7 cells. A specific aim of our research is to determine the optimal experimental conditions for RAW 264.7 cell growth and function. Our research will look at four experimental conditions present in existing peer-reviewed literature involving RAW 264.7 cells: 1) the use of DMEM vs. RPMI 1640 media, 2) the number of wells on a plate (24 or 96), 3) the number of cells added per well, and 4) the duration of cell exposure to LPS. We will evaluate which conditions are most popular in the existing scientific literature as well as compare the results from research using different experimental conditions. By determining the optimal experimental conditions for RAW 264.7 cells, we hope to produce a more standardized approach to experimental design for future experiments using this cell line.

Faculty Mentor(s)

Dr. David Freier

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Apr 8th, 12:00 PM Apr 8th, 1:15 PM

Determining the Optimal Experimental Conditions for RAW 264.7 Cells

Memorial Ballroom, Hall Campus Center

RAW 264.7 cells are murine macrophages derived from mice that are commonplace in microbiological research. There is a notable lack of consistency for the experimental conditions chosen by researchers working with this cell line. This variability makes it difficult to compare the results of scientific literature involving RAW 264.7 cells. A specific aim of our research is to determine the optimal experimental conditions for RAW 264.7 cell growth and function. Our research will look at four experimental conditions present in existing peer-reviewed literature involving RAW 264.7 cells: 1) the use of DMEM vs. RPMI 1640 media, 2) the number of wells on a plate (24 or 96), 3) the number of cells added per well, and 4) the duration of cell exposure to LPS. We will evaluate which conditions are most popular in the existing scientific literature as well as compare the results from research using different experimental conditions. By determining the optimal experimental conditions for RAW 264.7 cells, we hope to produce a more standardized approach to experimental design for future experiments using this cell line.