Virtual Presentations

Student Author Information

Haley Smeltz, University of LynchburgFollow

Location

Virtual Recording

Access Type

Open Access

Presentation Type

Oral Presentation

Start Date

8-4-2020 12:00 PM

End Date

8-4-2020 1:15 PM

Department

Chemistry

Abstract

Entomophagy, or the eating of insects, has been practiced in the eastern world for centuries, however it is currently overlooked in the western nations. Studies have shown that insects, such as crickets, contain high levels of protein and minerals, however vitamin content is rarely studied. This study aims to quantify the vitamin B12 content in two cricket species, the common house cricket (Acheta domesticus) and the banded cricket (Gryllodes sigillatus). The vitamin B12 content of both cricket species will be quantified using reverse-phase high performance liquid chromatography (RP-HPLC). Prior to RP-HPLC analysis, all the variants, or vitamers, of vitamin B12 in cricket samples will be converted into the cyanocobalamin variant, following by purification using B12 immunoaffinity column. At the conclusion of this study, the vitamin B12 content of both cricket species will be determined.

Faculty Mentor(s)

Dr. Samrat Thapa

Rights Statement

The right to download or print any portion of this material is granted by the copyright owner only for personal or educational use. The author/creator retains all proprietary rights, including copyright ownership. Any editing, other reproduction or other use of this material by any means requires the express written permission of the copyright owner. Except as provided above, or for any other use that is allowed by fair use (Title 17, §107 U.S.C.), you may not reproduce, republish, post, transmit or distribute any material from this web site in any physical or digital form without the permission of the copyright owner of the material.

Share

COinS
 
Apr 8th, 12:00 PM Apr 8th, 1:15 PM

Quantification of vitamin B12 in Two Cricket Species

Virtual Recording

Entomophagy, or the eating of insects, has been practiced in the eastern world for centuries, however it is currently overlooked in the western nations. Studies have shown that insects, such as crickets, contain high levels of protein and minerals, however vitamin content is rarely studied. This study aims to quantify the vitamin B12 content in two cricket species, the common house cricket (Acheta domesticus) and the banded cricket (Gryllodes sigillatus). The vitamin B12 content of both cricket species will be quantified using reverse-phase high performance liquid chromatography (RP-HPLC). Prior to RP-HPLC analysis, all the variants, or vitamers, of vitamin B12 in cricket samples will be converted into the cyanocobalamin variant, following by purification using B12 immunoaffinity column. At the conclusion of this study, the vitamin B12 content of both cricket species will be determined.