Poster or Presentation Title

Determining the Optimal Experimental Conditions for RAW 264.7 Cells

Location

Virtual | Room 4

Access Type

Campus Access Only

Presentation Type

Oral Presentation

Start Date

7-4-2021 9:15 AM

End Date

7-4-2021 9:30 AM

Abstract

RAW 264.7 cells are murine macrophages derived from mice that are a research model. There is a notable lack of consistency for the experimental conditions chosen by researchers working with this cell line. This variability makes it difficult to compare the results of scientific literature involving RAW 264.7 cells. A specific aim of our research is to determine the optimal experimental conditions for RAW 264.7 cell growth and function through literature review and experimentation. Our research looks at the effect of scraping versus trypsinization passage methods and the experience of the researcher on cellular response. A common stimulus of these RAW 264.7 cells is lipopolysaccharide (LPS) so an LPS dose-response was used as a comparative measure. We also evaluated which conditions are most popular in the existing scientific literature as well as compared the results from research using different experimental conditions. Our initial results suggest trypsin is a more consistent method for passage than scraping based on cell viability and nitric oxide (NO) response.

Faculty Mentor(s)

Dr. David Freier

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Apr 7th, 9:15 AM Apr 7th, 9:30 AM

Determining the Optimal Experimental Conditions for RAW 264.7 Cells

Virtual | Room 4

RAW 264.7 cells are murine macrophages derived from mice that are a research model. There is a notable lack of consistency for the experimental conditions chosen by researchers working with this cell line. This variability makes it difficult to compare the results of scientific literature involving RAW 264.7 cells. A specific aim of our research is to determine the optimal experimental conditions for RAW 264.7 cell growth and function through literature review and experimentation. Our research looks at the effect of scraping versus trypsinization passage methods and the experience of the researcher on cellular response. A common stimulus of these RAW 264.7 cells is lipopolysaccharide (LPS) so an LPS dose-response was used as a comparative measure. We also evaluated which conditions are most popular in the existing scientific literature as well as compared the results from research using different experimental conditions. Our initial results suggest trypsin is a more consistent method for passage than scraping based on cell viability and nitric oxide (NO) response.