Examination of C8-D1a Astrocytes to Pro-inflammatory Stimuli
Location
Room 232, Schewel Hall
Access Type
Open Access
Presentation Type
Oral presentation
Entry Number
2376
Start Date
4-16-2025 8:45 AM
End Date
4-16-2025 9:00 AM
School
School of Liberal Arts and Sciences
Department
Biology
Keywords
Neuroinflammation, Astrocytes, Nitric oxide, cytokines, Lipopolysaccharide, TNF-a, glial cells, Toll-like Receptor Pathways, NF-𝜅B signaling pathway, myD88
Abstract
Neuroinflammation is a complex synergy of many different cells within the central nervous system. Astrocytes play a key role in the maintenance of neural functioning, but take a special part in the inflammatory process as well. By releasing pro-inflammatory modulators like cytokines, chemokines, and reactive oxygen species, astrocytes modulate the inflammatory response in the brain. Astrocytes can be activated into this proinflammatory state in response to pathogenic molecules such as bacterial lipopolysaccharides (LPS). Lipopolysaccharides are glycoproteins derived from gram negative bacteria and commonly used to stimulate cells in vitro to produce an inflammatory response. To look at the effects of LPS on astrocyte inflammation, C8-D1a neonatal murine astrocytes were stimulated dose responsively from 0 - 1000 ng/mL. No significant change in nitric oxide production as measured by the Greiss reaction or in the pro-inflammatory cytokine TNF-ɑ was observed through multiple trials. These results are inconsistent within the published literature on astrocyte inflammation, thus more exploration is necessary to deduce the true effects of bacterial stimulation on astrocytes.
Primary Faculty Mentor(s)
Dr. David Freier
Primary Faculty Mentor(s) Department
Biology
Additional Faculty Mentor(s)
Dr. Ei Hlaing Dr. Price Blair
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Examination of C8-D1a Astrocytes to Pro-inflammatory Stimuli
Room 232, Schewel Hall
Neuroinflammation is a complex synergy of many different cells within the central nervous system. Astrocytes play a key role in the maintenance of neural functioning, but take a special part in the inflammatory process as well. By releasing pro-inflammatory modulators like cytokines, chemokines, and reactive oxygen species, astrocytes modulate the inflammatory response in the brain. Astrocytes can be activated into this proinflammatory state in response to pathogenic molecules such as bacterial lipopolysaccharides (LPS). Lipopolysaccharides are glycoproteins derived from gram negative bacteria and commonly used to stimulate cells in vitro to produce an inflammatory response. To look at the effects of LPS on astrocyte inflammation, C8-D1a neonatal murine astrocytes were stimulated dose responsively from 0 - 1000 ng/mL. No significant change in nitric oxide production as measured by the Greiss reaction or in the pro-inflammatory cytokine TNF-ɑ was observed through multiple trials. These results are inconsistent within the published literature on astrocyte inflammation, thus more exploration is necessary to deduce the true effects of bacterial stimulation on astrocytes.