Date Presented

Spring 5-15-2022

Document Type


Degree Name

Bachelor of Science


Biomedical Science

First Advisor

Dr. Aubrey Plourde

Second Advisor

Dr. Christine Terry

Third Advisor

Dr. David Freier


RAW 264.7 cells are an immortalized line of mouse cells that are commonly used in immunological research. They will be used to compare against murine bone marrow-derived macrophages (BMDM) to see if the two lines of cells are experimentally comparable in basic inflammatory testing. A standard method of measuring cell activity is through the analysis of nitric oxide production, which can be accomplished using a Griess reaction. Cell counts will be standardized to account for differing growth rates between the two cell lines. A similar output of nitric oxide concentration as an indicator of cellular activity between the RAW 264.7 cells and the BMDM will lead to the decreased need of animal testing and cellular extraction for inflammation research.