Oral Presentations
Location
Schewel Hall Room 222
Access Type
Event
Event Website
http://www.lynchburg.edu/academics/red-letter-day/student-scholar-showcase/
Entry Number
091
Start Date
4-6-2016 9:00 AM
End Date
4-6-2016 9:15 AM
Abstract
he examination of microbiomes has rapidly developed into a major field of study. The composition of oral flora of felines has been assumed to be similar to other mammalian species, but has not been extensively examined. Pure colonies collected by aseptic supragingival swab from three Felines, Felis domesticus, were investigated to determine bacterial species by means of biochemical and genomic methods. Initial culture of swabs in thioglycollate broth were utilized for the selection of anaerobic bacteria and then used to streak Columbia agar, Columbia blood agar, Mitis Salivarius agar and Anaerobe agar for 24 hour culture at 37°C under anaerobic conditions. Preliminary findings have revealed distinct, isolated colonies of common oral bacteria found on the streak plates including such species as Streptococcus mutans and Staphylococcus aureus. Under certain conditions these two organisms are known to contribute to pathogenesis. Ongoing work is being done to confirm initial findings, and to support a better understanding of the feline oral microbiome as an important factor in veterinary feline oral hygiene. Future research can be focused on the ecological benefits of the oral environment specifically with a focus on gram-positive, anaerobic bacteria in the oral micro-environment.
Faculty Mentor(s)
Dr. David O. Freier
The Isolation, Biochemical and Growth Characterization of Feline Supragingival Samples
Schewel Hall Room 222
he examination of microbiomes has rapidly developed into a major field of study. The composition of oral flora of felines has been assumed to be similar to other mammalian species, but has not been extensively examined. Pure colonies collected by aseptic supragingival swab from three Felines, Felis domesticus, were investigated to determine bacterial species by means of biochemical and genomic methods. Initial culture of swabs in thioglycollate broth were utilized for the selection of anaerobic bacteria and then used to streak Columbia agar, Columbia blood agar, Mitis Salivarius agar and Anaerobe agar for 24 hour culture at 37°C under anaerobic conditions. Preliminary findings have revealed distinct, isolated colonies of common oral bacteria found on the streak plates including such species as Streptococcus mutans and Staphylococcus aureus. Under certain conditions these two organisms are known to contribute to pathogenesis. Ongoing work is being done to confirm initial findings, and to support a better understanding of the feline oral microbiome as an important factor in veterinary feline oral hygiene. Future research can be focused on the ecological benefits of the oral environment specifically with a focus on gram-positive, anaerobic bacteria in the oral micro-environment.
https://digitalshowcase.lynchburg.edu/studentshowcase/2016/Presentations/10