Posters
Co-occurrence of Manganese oxidase genes
Location
Hall Memorial Ballroom
Access Type
Campus Access Only
Start Date
4-4-2018 12:00 PM
Department
Biology
Abstract
In the environment, manganese exists mostly in three oxidation states, Mn(II), Mn(III) and Mn(IV). Manganese oxidizing bacteria play a role in the environmental availability of Manganese in different oxidation states. Pseudomonas putida GB-1 is a model organism for the study of manganese oxidation in bacteria, however, the frequency of co-localization of multiple known and suspected manganese oxidizing proteins, as well as their occurrence between species, is unknown. Eight different genes isolated from known manganese oxidizing bacteria (MnxG, MopA, McoA, PputGB1_2552, PputGB1_2553, MoxA, MofA, and Bacillus MnxG) were tested individually using BioPython and BLAST (Basic Local Alignment Search Tool) on multiple genomic databases. BLAST searches had an expect value cutoff of at least 1e-20, with most searches limited with an expect value cutoff of 1e-50. The data from these BLAST searches was compiled into multiple matrices and used to calculate gene correlation. In fellow members of gamma-proteobacteria, correlations between MnxG, MopA, McoA, PputGB1_2552, and PputGB1_2553 were high, while across all species and between MoxA, MofA, and Bacillus MnxG, correlations were low in exception to the correlation between PputGB1_2552 and PputGB1_2553. The correlation between PputGB1_2552 and PputGB1_2553 in all instances is incredibly high. Further research is being conducted to identify a correlation between genes and environment. These correlations can be used in future research to determine the role of suspected manganese oxidizing proteins.
Faculty Mentor(s)
Katherine Geszvain, Mohamed Zaka Kurdi
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Co-occurrence of Manganese oxidase genes
Hall Memorial Ballroom
In the environment, manganese exists mostly in three oxidation states, Mn(II), Mn(III) and Mn(IV). Manganese oxidizing bacteria play a role in the environmental availability of Manganese in different oxidation states. Pseudomonas putida GB-1 is a model organism for the study of manganese oxidation in bacteria, however, the frequency of co-localization of multiple known and suspected manganese oxidizing proteins, as well as their occurrence between species, is unknown. Eight different genes isolated from known manganese oxidizing bacteria (MnxG, MopA, McoA, PputGB1_2552, PputGB1_2553, MoxA, MofA, and Bacillus MnxG) were tested individually using BioPython and BLAST (Basic Local Alignment Search Tool) on multiple genomic databases. BLAST searches had an expect value cutoff of at least 1e-20, with most searches limited with an expect value cutoff of 1e-50. The data from these BLAST searches was compiled into multiple matrices and used to calculate gene correlation. In fellow members of gamma-proteobacteria, correlations between MnxG, MopA, McoA, PputGB1_2552, and PputGB1_2553 were high, while across all species and between MoxA, MofA, and Bacillus MnxG, correlations were low in exception to the correlation between PputGB1_2552 and PputGB1_2553. The correlation between PputGB1_2552 and PputGB1_2553 in all instances is incredibly high. Further research is being conducted to identify a correlation between genes and environment. These correlations can be used in future research to determine the role of suspected manganese oxidizing proteins.