Presentations
Location
Schewel 232
Access Type
Open Access
Entry Number
128
Start Date
4-4-2018 1:15 PM
Department
Biology
Abstract
One in eight women in the United States will be diagnosed with breast cancer. Approximately 25-30% of these breast tumors will overexpress Human Epidermal Growth Factor Receptor 2 (HER2), associated with a worse prognosis and decreased survival rate. Stimulation of HER2 results in activation and production of many signaling molecules, ultimately resulting in increased cell division. Two of the downstream molecules activated are PI3Kinase and, further downstream, the molecule Akt. Typically, overproduction and/or overactivation of PI3Kinase can lead to oncogenesis. A molecule that may have a significant role in PI3Kinase formation is VAV1. VAV1 is produced via the stimulation of the Epidermal Growth Factor Receptor, a family member of HER2. VAV1 may mediate PI3Kinase’s ability to further activate downstream pathways. This study utilized small-interfering RNA (siRNA) to “knock-down” expression of VAV1. Once VAV1 is not expressed, activation of PI3Kinase and Akt can be tested to determine the necessity of VAV1 in this signaling pathway. Effects of the knock-down of VAV1 will be assessed using Western blotting with antibodies identifying phosphorylated PI3 Kinase, an indicator of activation.
PI3Kinase and Akt can be tested to determine the necessity of VAV1 in this signaling pathway. Effects of the knock-down of VAV1 will be assessed using Western blotting with antibodies identifying phosphorylated PI3 Kinase, an indicator of activation.
Faculty Mentor(s)
Dr. Jablonski
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Included in
siRNA Knock-down of Vav1 in Breast Cancer Cells: Effects on PI3Kinase and Akt
Schewel 232
One in eight women in the United States will be diagnosed with breast cancer. Approximately 25-30% of these breast tumors will overexpress Human Epidermal Growth Factor Receptor 2 (HER2), associated with a worse prognosis and decreased survival rate. Stimulation of HER2 results in activation and production of many signaling molecules, ultimately resulting in increased cell division. Two of the downstream molecules activated are PI3Kinase and, further downstream, the molecule Akt. Typically, overproduction and/or overactivation of PI3Kinase can lead to oncogenesis. A molecule that may have a significant role in PI3Kinase formation is VAV1. VAV1 is produced via the stimulation of the Epidermal Growth Factor Receptor, a family member of HER2. VAV1 may mediate PI3Kinase’s ability to further activate downstream pathways. This study utilized small-interfering RNA (siRNA) to “knock-down” expression of VAV1. Once VAV1 is not expressed, activation of PI3Kinase and Akt can be tested to determine the necessity of VAV1 in this signaling pathway. Effects of the knock-down of VAV1 will be assessed using Western blotting with antibodies identifying phosphorylated PI3 Kinase, an indicator of activation.
PI3Kinase and Akt can be tested to determine the necessity of VAV1 in this signaling pathway. Effects of the knock-down of VAV1 will be assessed using Western blotting with antibodies identifying phosphorylated PI3 Kinase, an indicator of activation.