Oral Presentations
Direct Acute Effects of Caffeine on RAW 264.7 Murine Macrophages Response to Inflammatory Stimulus by Bacterial Lipopolysaccharide.
Location
Room 232, Schewel Hall
Access Type
Campus Access Only
Entry Number
54
Start Date
4-10-2019 11:15 AM
End Date
4-10-2019 11:30 AM
College
Lynchburg College of Arts and Sciences
Department
Biomedical Science
Abstract
There are mixed findings on the effects of caffeine in human and animal experiments. The amount of caffeine consumed may determine the behavior of cellular and molecular inflammatory responses. Direct, in vitro studies, suggest caffeine may have anti-inflammatory effects. Previous unpublished work in this lab suggested pre-treatment of macrophages with caffeine may reduce nitric oxide responses to bacterial lipopolysaccharide (LPS) from E. coli. RAW 264.7 macrophages were seeded in 24-well plates at 4x105 cells per well in a 500 uL of DMEM complete. After acclimation overnight, wells are treated with caffeine at 0, 50, 100, and 500 uM concentrations for 24 hours. Cells are co-stimulated with either 1ng/mL or 100ng/mL of LPS for 24 hours. Nitrites in solution, as a measure of inducible nitric oxide synthase (iNOS) activity, are determined by the Greiss Reaction to determine the level of inflammatory activity of the RAW 264.7 cells. Additionally, pre-treatment studies with caffeine are also planned. Our analysis may allow for a better understanding of what effect caffeine has in modulating the inflammatory response.
Faculty Mentor(s)
Dr. David O. Freier
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Direct Acute Effects of Caffeine on RAW 264.7 Murine Macrophages Response to Inflammatory Stimulus by Bacterial Lipopolysaccharide.
Room 232, Schewel Hall
There are mixed findings on the effects of caffeine in human and animal experiments. The amount of caffeine consumed may determine the behavior of cellular and molecular inflammatory responses. Direct, in vitro studies, suggest caffeine may have anti-inflammatory effects. Previous unpublished work in this lab suggested pre-treatment of macrophages with caffeine may reduce nitric oxide responses to bacterial lipopolysaccharide (LPS) from E. coli. RAW 264.7 macrophages were seeded in 24-well plates at 4x105 cells per well in a 500 uL of DMEM complete. After acclimation overnight, wells are treated with caffeine at 0, 50, 100, and 500 uM concentrations for 24 hours. Cells are co-stimulated with either 1ng/mL or 100ng/mL of LPS for 24 hours. Nitrites in solution, as a measure of inducible nitric oxide synthase (iNOS) activity, are determined by the Greiss Reaction to determine the level of inflammatory activity of the RAW 264.7 cells. Additionally, pre-treatment studies with caffeine are also planned. Our analysis may allow for a better understanding of what effect caffeine has in modulating the inflammatory response.