Poster or Presentation Title

Direct Acute Effects of Caffeine on RAW 264.7 Murine Macrophages Response to Inflammatory Stimulus by Bacterial Lipopolysaccharide.

Student Author Information

Abigail KaufmannFollow

Access Type

Campus Access Only

Presentation Type

Oral Presentation

Start Date

April 2019

Department

Biomedical Science

Abstract

There are mixed findings on the effects of caffeine in human and animal experiments. The amount of caffeine consumed may determine the behavior of cellular and molecular inflammatory responses. Direct, in vitro studies, suggest caffeine may have anti-inflammatory effects. Previous unpublished work in this lab suggested pre-treatment of macrophages with caffeine may reduce nitric oxide responses to bacterial lipopolysaccharide (LPS) from E. coli. RAW 264.7 macrophages were seeded in 24-well plates at 4x105 cells per well in a 500 uL of DMEM complete. After acclimation overnight, wells are treated with caffeine at 0, 50, 100, and 500 uM concentrations for 24 hours. Cells are co-stimulated with either 1ng/mL or 100ng/mL of LPS for 24 hours. Nitrites in solution, as a measure of inducible nitric oxide synthase (iNOS) activity, are determined by the Greiss Reaction to determine the level of inflammatory activity of the RAW 264.7 cells. Additionally, pre-treatment studies with caffeine are also planned. Our analysis may allow for a better understanding of what effect caffeine has in modulating the inflammatory response.

Faculty Mentor(s)

Dr. David O. Freier

Rights Statement

The right to download or print any portion of this material is granted by the copyright owner only for personal or educational use. The author/creator retains all proprietary rights, including copyright ownership. Any editing, other reproduction or other use of this material by any means requires the express written permission of the copyright owner. Except as provided above, or for any other use that is allowed by fair use (Title 17, §107 U.S.C.), you may not reproduce, republish, post, transmit or distribute any material from this web site in any physical or digital form without the permission of the copyright owner of the material.

This document is currently not available here.

Share

COinS
 
Apr 10th, 11:15 AM

Direct Acute Effects of Caffeine on RAW 264.7 Murine Macrophages Response to Inflammatory Stimulus by Bacterial Lipopolysaccharide.

There are mixed findings on the effects of caffeine in human and animal experiments. The amount of caffeine consumed may determine the behavior of cellular and molecular inflammatory responses. Direct, in vitro studies, suggest caffeine may have anti-inflammatory effects. Previous unpublished work in this lab suggested pre-treatment of macrophages with caffeine may reduce nitric oxide responses to bacterial lipopolysaccharide (LPS) from E. coli. RAW 264.7 macrophages were seeded in 24-well plates at 4x105 cells per well in a 500 uL of DMEM complete. After acclimation overnight, wells are treated with caffeine at 0, 50, 100, and 500 uM concentrations for 24 hours. Cells are co-stimulated with either 1ng/mL or 100ng/mL of LPS for 24 hours. Nitrites in solution, as a measure of inducible nitric oxide synthase (iNOS) activity, are determined by the Greiss Reaction to determine the level of inflammatory activity of the RAW 264.7 cells. Additionally, pre-treatment studies with caffeine are also planned. Our analysis may allow for a better understanding of what effect caffeine has in modulating the inflammatory response.