Access Type

Campus Access Only

Presentation Type

Oral Presentation

Start Date

April 2019



Faculty Mentor(s)

Dr. Pepper Hanna; Dr. Keith Corodimas


Previous research demonstrates that chronic and acute exposure to neonicotinoid insecticides, like chlorpyrifos, inhibits spatial learning in many vertebrates, but virtually nothing is currently known about the effects of neonicotinoids on emotional (fear-related) learning in non-mammalian vertebrates, including fish. The goal of the current study was to investigate the acute effects of chlorpyrifos, a ubiquitous insecticide, on the acquisition of contextual fear conditioning in zebrafish (Danio rerio). Adult zebrafish were randomly assigned to the following groups: 0.0 (placebo), 0.01, 0.1, 1.0 mg/L chlorpyrifos. After a 10-min drug or placebo exposure, fish received five contextual fear conditioning trials (20 mA/3-sec shocks) with 60-sec inter-trial intervals. Twenty-four hours later, conditioned fear responses were blindly observed and recorded (AquaScan video tracking software) in the original, conditioning context and in a novel context in a counterbalanced fashion. Fear was operationally defined as the number and duration of freezing bouts, as well as swim distance. It was hypothesized that chlorpyrifos will dose-dependently impair the acquisition of context-dependent, emotional learning compared with controls. Supported generously by Schewel & Wooten Funds.

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Apr 10th, 1:30 PM

The Effects of the Neonicotinoid, Chlorpyrifos, on Contextual Fear Conditioning in Adult Zebrafish (Danio rerio)