The Impact of Oxygen Exposure and pH on the Growth and Biofilm Formation of Veillonella Atypica, in Relation to Human Health
Location
Access Type
Campus Access Only
Entry Number
70
Start Date
4-7-2021 2:30 PM
End Date
4-7-2021 2:45 PM
Department
Biomedical Science
Abstract
Veillonella atypica is found in many microbiomes of the human body, including the gut and oral cavities. Previous studies have primarily focused on the role of V. atypica in the oral microbiome, its association with periodontal disease, and its role as an early colonizer in the development of dental biofilm. V. atypica is an extremely understudied bacteria that lives in the human microbiome and has known metabolic pathways that utilize lactate as a primary carbon source. Athletes have increased concentrations of lactate in circulation and V. atypica was found to be associated with enhanced athletic performance in endurance athletes. The aerotolerance of V. atypica was much higher than expected and the bacteria were still living after 24 hours exposure to oxygen. V. atypica was grown in BHIL broth in a pH range of 2.85 to 11.85 and grew most successfully and had greatest biofilm production in the pH range of 5.85 to 9.85. This range correlates with the pH range of the small and large intestine of the human gastrointestinal (GI) tract. Additional study on the impact of lactate concentration, aerotolerance, and biofilm contribution in the gut could provide a more definitive link between V. atypica and human health.
Faculty Mentor(s)
Dr. Jamie Brooks
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The Impact of Oxygen Exposure and pH on the Growth and Biofilm Formation of Veillonella Atypica, in Relation to Human Health
Veillonella atypica is found in many microbiomes of the human body, including the gut and oral cavities. Previous studies have primarily focused on the role of V. atypica in the oral microbiome, its association with periodontal disease, and its role as an early colonizer in the development of dental biofilm. V. atypica is an extremely understudied bacteria that lives in the human microbiome and has known metabolic pathways that utilize lactate as a primary carbon source. Athletes have increased concentrations of lactate in circulation and V. atypica was found to be associated with enhanced athletic performance in endurance athletes. The aerotolerance of V. atypica was much higher than expected and the bacteria were still living after 24 hours exposure to oxygen. V. atypica was grown in BHIL broth in a pH range of 2.85 to 11.85 and grew most successfully and had greatest biofilm production in the pH range of 5.85 to 9.85. This range correlates with the pH range of the small and large intestine of the human gastrointestinal (GI) tract. Additional study on the impact of lactate concentration, aerotolerance, and biofilm contribution in the gut could provide a more definitive link between V. atypica and human health.