Location

Room 232, Schewel Hall

Access Type

Campus Access Only

Start Date

4-17-2024 8:15 AM

End Date

4-17-2024 8:30 AM

College

College of Health Sciences

Department

Biomedical Science

Abstract

Currently, there is virtually no research done for the oral microbiome of a llama. There are articles about the gut microbiome of alpacas and other ruminants, which in theory should be similar to that of a llama because they are often housed and cared for in a very similar manner. The gut and oral microbiome in most mammals have proven to be similar, especially in animals that chew cud like llamas and cows. In a study of the gut microbiome of ruminants, there are several members that appear across species including these bacteria groups Prevotella, Butyrivibrio, and Ruminococcus, as well as unclassified Lachnospiraceae, Ruminococcaceae, Bacteroidales, and Clostridiales which appeared in 90% of the animals surveyed. In ruminants these are considered the “core bacterial microbiome”. They have also found Methanobrevibacter gottschalkii and Methanobrevibacter ruminantium, which account for 74% of the archaea found (Henderson et.al). This project will be submitting samples from three different llamas for 16s rRNA testing to identify and sequence the oral microbiome of each. It will also be isolating a single microbe from a sample to identify it using biochemical testing. The oral microbiome is important to understand because it can tell a lot about the living conditions of the animal. It is also important for the handlers to understand what is in the oral microbiome and the implications it could have on their health.

Faculty Mentor(s)

Dr. Jamie Brooks

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Apr 17th, 8:15 AM Apr 17th, 8:30 AM

Investigation of the Oral Microbiome of Llamas: Genomic Sequencing and Identification of an Isolated Microbe

Room 232, Schewel Hall

Currently, there is virtually no research done for the oral microbiome of a llama. There are articles about the gut microbiome of alpacas and other ruminants, which in theory should be similar to that of a llama because they are often housed and cared for in a very similar manner. The gut and oral microbiome in most mammals have proven to be similar, especially in animals that chew cud like llamas and cows. In a study of the gut microbiome of ruminants, there are several members that appear across species including these bacteria groups Prevotella, Butyrivibrio, and Ruminococcus, as well as unclassified Lachnospiraceae, Ruminococcaceae, Bacteroidales, and Clostridiales which appeared in 90% of the animals surveyed. In ruminants these are considered the “core bacterial microbiome”. They have also found Methanobrevibacter gottschalkii and Methanobrevibacter ruminantium, which account for 74% of the archaea found (Henderson et.al). This project will be submitting samples from three different llamas for 16s rRNA testing to identify and sequence the oral microbiome of each. It will also be isolating a single microbe from a sample to identify it using biochemical testing. The oral microbiome is important to understand because it can tell a lot about the living conditions of the animal. It is also important for the handlers to understand what is in the oral microbiome and the implications it could have on their health.