Date Presented
Spring 5-15-2022
Document Type
Thesis
Degree Name
Bachelor of Science
Department
Biomedical Science
First Advisor
Dr. Aubrey Plourde
Second Advisor
Dr. Christine Terry
Third Advisor
Dr. David Freier
Abstract
RAW 264.7 cells are an immortalized line of mouse cells that are commonly used in immunological research. They will be used to compare against murine bone marrow-derived macrophages (BMDM) to see if the two lines of cells are experimentally comparable in basic inflammatory testing. A standard method of measuring cell activity is through the analysis of nitric oxide production, which can be accomplished using a Griess reaction. Cell counts will be standardized to account for differing growth rates between the two cell lines. A similar output of nitric oxide concentration as an indicator of cellular activity between the RAW 264.7 cells and the BMDM will lead to the decreased need of animal testing and cellular extraction for inflammation research.
Recommended Citation
Harker, Quinn, "Functional Comparison of RAW 264.7 Cells and Murine Bone Marrow-Derived Macrophages (BMDM)" (2022). Undergraduate Theses and Capstone Projects. 238.
https://digitalshowcase.lynchburg.edu/utcp/238