Location

Schewel 232

Access Type

Open Access

Presentation Type

Oral Presentation

Start Date

April 2022

Department

Biomedical Science

Abstract

Echinacea purpurea, commonly known as the purple coneflower, is utilized in medicinal preparations due to potential immunostimulatory and anti-inflammatory properties. Present bioactive metabolites such as alkamides, polysaccharides, and caffeic acid derivatives provide pharmacological elements essential to mitigating symptoms produced by upper respiratory illnesses. The chemical composition of E. purpurea holds medicinal value, but there remains controversy surrounding the efficacy of its use in medicine. The purpose of this study is to examine the effects of an 75% ethanolic E. purpurea root extract on LPS (lipopolysaccharide)-stimulated RAW 264.7 murine macrophages. Treatment groups included ethanolic E. purpurea extract (3µL, 15µL, 30µL), negative control groups (only DMEM), positive control groups (100 ng/mL of LPS), and control groups for ethanol concentrations (3µL, 15µL, 30µL). A series of 5 experiments demonstrated a dose dependent decrease in nitrite concentrations with increasing E. purpurea extract. However, recent replicate experiments using newly prepared E. purpurea extract suggests that E. purpurea did not have an effect on nitrite concentrations. In future studies, chemical analysis through gas chromatography-mass spectrometry (GC-MS) is essential to determine chemical composition to verify the presence of bioactive elements.

Faculty Mentor(s)

David Freier

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Apr 6th, 11:15 AM

The Effect of Ethanolic Echinacea purpurea Root Extracts on the Production of Pro-Inflammatory Mediators in RAW 264.7 Murine Macrophages

Schewel 232

Echinacea purpurea, commonly known as the purple coneflower, is utilized in medicinal preparations due to potential immunostimulatory and anti-inflammatory properties. Present bioactive metabolites such as alkamides, polysaccharides, and caffeic acid derivatives provide pharmacological elements essential to mitigating symptoms produced by upper respiratory illnesses. The chemical composition of E. purpurea holds medicinal value, but there remains controversy surrounding the efficacy of its use in medicine. The purpose of this study is to examine the effects of an 75% ethanolic E. purpurea root extract on LPS (lipopolysaccharide)-stimulated RAW 264.7 murine macrophages. Treatment groups included ethanolic E. purpurea extract (3µL, 15µL, 30µL), negative control groups (only DMEM), positive control groups (100 ng/mL of LPS), and control groups for ethanol concentrations (3µL, 15µL, 30µL). A series of 5 experiments demonstrated a dose dependent decrease in nitrite concentrations with increasing E. purpurea extract. However, recent replicate experiments using newly prepared E. purpurea extract suggests that E. purpurea did not have an effect on nitrite concentrations. In future studies, chemical analysis through gas chromatography-mass spectrometry (GC-MS) is essential to determine chemical composition to verify the presence of bioactive elements.