Location
Sydnor Performance Hall, Schewel Hall
Access Type
Campus Access Only
Presentation Type
Oral presentation
Entry Number
94
Start Date
4-16-2026 3:30 PM
End Date
4-16-2026 3:45 PM
School
School of Liberal Arts and Sciences
Department
Biology
Keywords
Macrophage, BMDM, RAW 264.7, DMEM, RPMI 1640
Abstract
Macrophages play a vital role in the innate immune response by responding to pathogen-associated molecular patterns (PAMPs) and triggering a pro-inflammatory response. Multiple macrophage cell lines are used for macrophage pro-inflammatory research, but little is known about whether their responses are similar across multiple types of culture media. Previous studies show that the RAW 264.7 macrophage line produces nitric oxide (NO) in a dose-response fashion when grown in high glucose (HG) DMEM and high glucose (HG) RPMI 1640, but responds poorly when grown in standard RPMI 1640 (glucose at 2.0 g/L). However, responses by bone marrow-derived macrophages (BMDM) in different culture media remain unexplored. A direct comparison of the pro-inflammatory responses of BMDM and RAW 264.7 cells cultured in high glucose DMEM, low glucose RPMI 1640 and high glucose RPMI 1640 to bacterial lipopolysaccharide (LPS) stimulation will evaluate both macrophage cell types in equivalent conditions. BMDMs were developed from hematopoietic stem cells collected from the bone marrow of female Swiss mice. BMDM cells and RAW 264.7 macrophages are cultured in 24-well plates at 4x105 cells/well in 500 μL of culture medium. At 24 hours after dose-responsive LPS stimulation (0 – 100 ng/mL), nitric oxide responses were measured using Griess reagent and spectrophotometry assays. Both BMDM and RAW 264.7 cells displayed a dose-response pattern to LPS across all media types. RAW 264.7 cells in DMEM produced the highest nitric oxide concentration, while BMDMs were unable to produce similar responses to RAW 264.7 cells across all media types. This suggests that BMDMs produce lower levels of pro-inflammatory mediators that are independent of glucose concentration but could be due to functional differences or growth rate of the cells.
Primary Faculty Mentor(s)
Dr. David O Freier
Primary Faculty Mentor(s) Department
Biology
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Effect of Culture Media on Bone Marrow Derived Macrophage Response
Sydnor Performance Hall, Schewel Hall
Macrophages play a vital role in the innate immune response by responding to pathogen-associated molecular patterns (PAMPs) and triggering a pro-inflammatory response. Multiple macrophage cell lines are used for macrophage pro-inflammatory research, but little is known about whether their responses are similar across multiple types of culture media. Previous studies show that the RAW 264.7 macrophage line produces nitric oxide (NO) in a dose-response fashion when grown in high glucose (HG) DMEM and high glucose (HG) RPMI 1640, but responds poorly when grown in standard RPMI 1640 (glucose at 2.0 g/L). However, responses by bone marrow-derived macrophages (BMDM) in different culture media remain unexplored. A direct comparison of the pro-inflammatory responses of BMDM and RAW 264.7 cells cultured in high glucose DMEM, low glucose RPMI 1640 and high glucose RPMI 1640 to bacterial lipopolysaccharide (LPS) stimulation will evaluate both macrophage cell types in equivalent conditions. BMDMs were developed from hematopoietic stem cells collected from the bone marrow of female Swiss mice. BMDM cells and RAW 264.7 macrophages are cultured in 24-well plates at 4x105 cells/well in 500 μL of culture medium. At 24 hours after dose-responsive LPS stimulation (0 – 100 ng/mL), nitric oxide responses were measured using Griess reagent and spectrophotometry assays. Both BMDM and RAW 264.7 cells displayed a dose-response pattern to LPS across all media types. RAW 264.7 cells in DMEM produced the highest nitric oxide concentration, while BMDMs were unable to produce similar responses to RAW 264.7 cells across all media types. This suggests that BMDMs produce lower levels of pro-inflammatory mediators that are independent of glucose concentration but could be due to functional differences or growth rate of the cells.